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lee via addgene  (Addgene inc)


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    Structured Review

    Addgene inc lee via addgene
    Lee Via Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pjbei+6409+plasmid/pJBEI-6409+(Plasmid+%2347048)/us12385841-434-15-17
    Average 93 stars, based on 20 article reviews
    lee via addgene - by Bioz Stars, 2026-09
    93/100 stars

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    Plasmid Preparation:

    Article Title: Enhancing limonene production by probing the metabolic network through time-series metabolomics data.
    Article Snippet: Water was deionised and filtered by Sartorius Arium Pro VF Type 1 water system (18.2 MΩ, 0.2 μm). .. The limonene producing engineered wild-type strain was created by transforming pJBEI-6409 plasmid which was a gift from Taek Soon Lee (Addgene plasmid #47048; http:// n2t.net/addgene:47048; RRID: Addgene_47048) (AlonsoGutierrez et al., 2013) by heat shock in E. coli K-12 MG1655 (F- lambda- ilvG- rfb-50 rph-1) forming the strain EcoCTs3. ..

    Article Title: Exploring Natural Diversity of Limonene Synthases and Molecular Determinants Involved in Substrate Specificity in Escherichia coli .
    Article Snippet: Limonene is a chiral, high-demand monoterpene that has wide applications in therapeutics, cosmetics, biofuels, agrifood, biomaterials, and solvent industries.. However, its biosynthesis by microbial cell factories is often limited by the poor activity of limonene synthase (LS).. Optimization of the rate-limiting enzyme is thus crucial for boosting limonene production.

    Article Title: Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli.
    Article Snippet: Deionised water was filtered by Sartorius Arium Pro VF Type 1 water system (18.2MΩ, 0.2 μm). .. The engineered wild-type strain producing limonene, EcoCTs03, was produced by transforming pJBEI-6409 plasmid, which was a gift fromTaek Soon Lee (Addgene plasmid # 47048; http://n2t.net/addgene:47048; RRID:Addgene_47048)3 throughheat shock inE. coliK-12MG1655. pJBEI6409 is a p15A plasmid expressing limonene from acetyl-CoA via geranyl pyrophosphate (GPP) and was used as-is in this study. ..

    Article Title: Enhancing Limonene production by probing the metabolic network through time-series metabolomics data
    Article Snippet: .. Strain construction The limonene producing engineered wild-type strain was created by transforming pJBEI-6409 plasmid which was a gift from Taek Soon Lee (Addgene plasmid #47048 ; http://n2t.net/addgene:47048 ; RRID:Addgene_47048) (19) by heat shock in E. coli K-12 MG1655 (F- lambda- ilvG- rfb-50 rph-1) forming the strain EcoCTs3. ..

    Article Title: Enhanced Production of (+)-Limonene through Targeted Engineering of Citrus sinensis Limonene Synthase.
    Article Snippet: Get e-Alerts RESEARCH ARTICLE | May 28, 2025 Enhanced Production of (+)-Limonene through Targeted Engineering of Citrus sinensis Limonene Synthase , , , , , , , , , , , , , and ACS Synthetic Biology Cite this: ACS Synth.. Biol.. 2025, XXXX, XXX, XXX-XXX https://doi.org/10.1021/acssynbio.5c00078 © 2025 American Chemical Society Request reuse permissions Cite Share Jump to Clement P. M. Scipion Jérémy Esque Shreyash Borkar Jing Sen Ong Cristalle Seah Leonard Ong Pnelope Ng Yoganathan Kanagasundaram Magali Remaud-Simeon Sophie Bozonnet Bo Xue Wen Shan Yew Isabelle André* Xixian Chen* Open PDF Supporting Information (1) Article Views 76 Altmetric Citations Learn about these metrics Published May 28, 2025 6/2/25, 2:44 PM Enhanced Production of (+)-Limonene through Targeted Engineering of Citrus sinensis Limonene Synthase | ACS Synthetic Biology https://pubs.acs.org/doi/10.1021/acssynbio.5c00078 2/27 Limonene is a high-value monoterpene with numerous industrial applications including avors, fragrances, and pharmaceuticals.

    Article Title: Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli
    Article Snippet: Deionised water was filtered by Sartorius Arium Pro VF Type 1 water system (18.2 MΩ, 0.2 μm). .. The engineered wild-type strain producing limonene, EcoCTs03, was produced by transforming pJBEI-6409 plasmid, which was a gift from Taek Soon Lee (Addgene plasmid # 47048; http://n2t.net/addgene:47048 ; RRID:Addgene_47048) through heat shock in E. coli K-12 MG1655. pJBEI-6409 is a p15A plasmid expressing limonene from acetyl-CoA via geranyl pyrophosphate (GPP) and was used as-is in this study. ..

    Produced:

    Article Title: Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli.
    Article Snippet: Deionised water was filtered by Sartorius Arium Pro VF Type 1 water system (18.2MΩ, 0.2 μm). .. The engineered wild-type strain producing limonene, EcoCTs03, was produced by transforming pJBEI-6409 plasmid, which was a gift fromTaek Soon Lee (Addgene plasmid # 47048; http://n2t.net/addgene:47048; RRID:Addgene_47048)3 throughheat shock inE. coliK-12MG1655. pJBEI6409 is a p15A plasmid expressing limonene from acetyl-CoA via geranyl pyrophosphate (GPP) and was used as-is in this study. ..

    Article Title: Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli
    Article Snippet: Deionised water was filtered by Sartorius Arium Pro VF Type 1 water system (18.2 MΩ, 0.2 μm). .. The engineered wild-type strain producing limonene, EcoCTs03, was produced by transforming pJBEI-6409 plasmid, which was a gift from Taek Soon Lee (Addgene plasmid # 47048; http://n2t.net/addgene:47048 ; RRID:Addgene_47048) through heat shock in E. coli K-12 MG1655. pJBEI-6409 is a p15A plasmid expressing limonene from acetyl-CoA via geranyl pyrophosphate (GPP) and was used as-is in this study. ..

    Expressing:

    Article Title: Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli.
    Article Snippet: Deionised water was filtered by Sartorius Arium Pro VF Type 1 water system (18.2MΩ, 0.2 μm). .. The engineered wild-type strain producing limonene, EcoCTs03, was produced by transforming pJBEI-6409 plasmid, which was a gift fromTaek Soon Lee (Addgene plasmid # 47048; http://n2t.net/addgene:47048; RRID:Addgene_47048)3 throughheat shock inE. coliK-12MG1655. pJBEI6409 is a p15A plasmid expressing limonene from acetyl-CoA via geranyl pyrophosphate (GPP) and was used as-is in this study. ..

    Article Title: Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli
    Article Snippet: Deionised water was filtered by Sartorius Arium Pro VF Type 1 water system (18.2 MΩ, 0.2 μm). .. The engineered wild-type strain producing limonene, EcoCTs03, was produced by transforming pJBEI-6409 plasmid, which was a gift from Taek Soon Lee (Addgene plasmid # 47048; http://n2t.net/addgene:47048 ; RRID:Addgene_47048) through heat shock in E. coli K-12 MG1655. pJBEI-6409 is a p15A plasmid expressing limonene from acetyl-CoA via geranyl pyrophosphate (GPP) and was used as-is in this study. ..



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